Lateral and axial resolution criteria in incoherent and coherent optics and holography, near- and far-field regimes

This work presents an overview of the spatial resolution criteria in classical optics, digital optics and holography. Although the classical Abbe and Rayleigh resolution criteria have been thoroughly discussed in the literature, there are still several issues which still need to be addressed, for example the axial resolution criterion for coherent and incoherent radiation, which is a crucial parameter of three-dimensional (3D) imaging, the resolution criteria in the Fresnel regime, and the lateral and axial resolution criteria in digital optics and holography. This work discusses these issues and provides a simple guide for which resolution criteria should be applied in each particular imaging scheme: coherent/incoherent, far- and near-field, lateral and axial resolution. Different resolution criteria such as two-point resolution and the resolution obtained from the image spectrum (diffraction pattern) are compared and demonstrated with simulated examples. Resolution criteria for spatial lateral and axial resolution are derived, and their application in imaging with coherent and incoherent (noncoherent) waves is considered. It is shown that for coherent light, the classical Abbe and Rayleigh resolution criteria do not provide an accurate estimation of the lateral and axial resolution. Lateral and axial resolution criteria based on an evaluation of the spectrum of the diffracted wave provide a more precise estimation of the resolution for coherent and incoherent light. It is also shown that resolution criteria derived in approximation of the far-field imaging regime can be applied for the near-field (Fresnel) regime

Reconstruction of missing information in diffraction patterns and holograms by iterative phase retrieval

It is demonstrated that an object distribution can be successfully retrieved from its diffraction pattern or hologram, even if some of the measured intensity samples are missing. The maximum allowable number of missing values depends on the linear oversampling ratio s, where the higher the value of s, the more intensity samples can be missing. For a real-valued object, the ratio of missing pixels to the total number of pixels should not exceed (1 - 2/s^2) or (1 - 1/s^2) in the acquired diffraction pattern or hologram, respectively. For example, even 5% of the measured intensity values at an oversampling ratio of s = 8 are sufficient to simultaneously retrieve the object distribution and the missing intensity values. It is important that the missing intensity values should not be concentrated in the centre, but should be randomly distributed over the acquired diffraction pattern

Three-dimensional double helical DNA structure directly revealed from its X-ray fiber diffraction pattern by iterative phase retrieval

Coherent diffraction imaging (CDI) allows the retrieval of the structure of an isolated object, such as a macromolecule, from its diffraction pattern. CDI requires the fulfilment of two conditions: the imaging radiation must be coherent and the object must be isolated. We discuss that it is possible to directly retrieve the molecular structure from its diffraction pattern which was acquired neither with coherent radiation nor from an individual molecule, provided the molecule exhibits periodicity in one direction, as in the case of fiber diffraction. We demonstrate that by applying iterative phase retrieval methods to a fiber diffraction pattern, the repeating unit, that is, the molecule structure, can directly be reconstructed without any prior modeling. As an example, we recover the structure of the DNA double helix in three-dimensions from its two-dimensional X-ray fiber diffraction pattern, Photograph 51, acquired in the famous experiment by Raymond Gosling and Rosalind Franklin, at a resolution of 3.4 Angstrom

Practical algorithms for simulation and reconstruction of digital in-line holograms

Here we present practical methods for simulation and reconstruction of in-line digital holograms recorded with plane and spherical waves. The algorithms described here are applicable to holographic imaging of an object exhibiting absorption as well as phase shifting properties. Optimal parameters, related to distances, sampling rate, and other factors for successful simulation and reconstruction of holograms are evaluated and criteria for the achievable resolution are worked out. Moreover, we show that the numerical procedures for the reconstruction of holograms recorded with plane and spherical waves are identical under certain conditions. Experimental examples of holograms and their reconstructions are also discussed.Comment: including MATLAB code

Spatial coherence of electron beams from field emitters and its effect on the resolution of imaged objects

Sub-nanometer and nanometer-sized tips provide high coherence electron sources. Conventionally, the effective source size is estimated from the extent of the experimental biprism interference pattern created on the detector by applying the van Cittert Zernike theorem. Previously reported experimental intensity distributions on the detector exhibit Gaussian distribution and our simulations show that this is an indication that such electron sources must be at least partially coherent. This, in turn means that strictly speaking the Van Cittert Zernike theorem cannot be applied, since it assumes an incoherent source. The approach of applying the van Cittert Zernike theorem is examined in more detail by performing simulations of interference patterns for the electron sources of different size and different coherence length, evaluating the effective source size from the extent of the simulated interference pattern and comparing the obtained result with the pre-defined value. The intensity distribution of the source is assumed to be Gaussian distributed, as it is observed in experiments. The visibility or the contrast in the simulated holograms is found to be always less than 1 which agrees well with previously reported experimental results and thus can be explained solely by the Gaussian intensity distribution of the source. The effective source size estimated from the extent of the interference pattern turns out to be of about 2-3 times larger than the pre-defined size, but it is approximately equal to the intrinsic resolution of the imaging system. A simple formula for estimating the intrinsic resolution, which could be useful when employing nano-tips in in-line Gabor holography or point-projection microscopy, is provided

The role of the coherence in the cross-correlation analysis of diffraction patterns from two-dimensional dense mono-disperse systems

The investigation of the static and dynamic structural properties of colloidal systems relies on techniques capable of atomic resolution in real space and femtosecond resolution in time. Recently, the cross-correlation function (CCF) analysis of both X-rays and electron diffraction patterns from dilute and dense aggregates has demonstrated the ability to retrieve information on the sample's local order and symmetry. Open questions remain regarding the role of the beam coherence in the formation of the diffraction pattern and the properties of the CCF, especially in dense systems. Here, we simulate the diffraction patterns of dense two-dimensional monodisperse systems of different symmetries, varying the transverse coherence of the probing wave, and analyze their CCF. We study samples with different symmetries at different size scale, as for example, pentamers arranged into a four-fold lattice where each pentamer is surrounded by triangular lattices, both ordered and disordered. In such systems, different symmetry modulations are arising in the CCF at specific scattering vectors. We demonstrate that the amplitude of the CCF is a fingerprint of the degree of the ordering in the sample and that at partial transverse coherence, the CCF of a dense sample corresponds to that of an individual scattering object.Comment: 22 pages, 7 figure